Gibco™ CD CHO AGT™ Medium

Catalog No.	Quantity
12-490-017	1 L
12-490-025	10 L
12-490-001	100 L

Catalog No. 12-490-017 Supplier Gibco™ Supplier No. 12490017

Description

CD CHO AGT™ Medium

CD CHO AGT™ (Advanced Granulation Technology) medium is a protein-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. The AGT™ dry media format is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing. CD CHO AGT™ medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates.

When to Use CD CHO Medium

Your GS CHO cells are recommended to be grown in CD CHO medium

Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

When to Consider Another Gibco™ Medium

Consider CD FortiCHO™ medium when:

The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line

Maximum batch culture cell densities and protein titers are needed

You have time to adapt cells into a new medium in an effort to maximize titers

Cell health at harvest is a priority for downstream processing

Consider CD OptiCHO™ medium when:

You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines

Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity

Your CHO cell line is finicky, i.e. hard to grow or adapt

Consider CD DG44 medium when:

You use parental DG44 or DXB11 CHO cells (dhfr-)

Liquid Media Formulations
Gibco™ AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco™ liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco™ cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Specifications

• Cell Line: CHO
• Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
• Form: Powder (AGT)
• Product Type: CD CHO Medium
• Serum Level: Serum-free
• With Additives: Sodium Bicarbonate
• Without Additives: No Glutamine, No Phenol Red, No Sodium Pyruvate, No Thymidine, No Hypoxanthine
• Product Line: AGT, Gibco
• Quantity: 1 L
• Shipping Condition: Wet Ice
• Endotoxin Level: ≤10 EU/mL, Low
• Format: Securitainer Capped Vial
• Included Antibiotics: No antibiotics
• Reconstituted Volume: 1 L
• Species: Hamster
• Content And Storage: Store in refrigerator (2–8°C).
• Culture Type: Suspension Cell Culture

Frequently Asked Questions (FAQs)

Which serum-free medium should I select for my CHO cells?

Thermo Fisher Scientific offers multiple serum free media (SFM) and chemically defined (CD) media options for CHO cultures. For adherent cultures, CD CHO (10743-011) and CD OptiCHO medium (12681-011) are robust media; both are protein free and animal origin free (AOF). For adherent cultures, CD CHO-A (097-0182DJ) is a widely used protein free and animal origin free medium. There are other formulations available to suit other research needs; contact Technical Support for further information.

What are the main benefits of using AGT?

Complete formulations
- Homogeneous distribution of minute components
- Complete complex formulations in a dry format, including serum-free, protein-free, and chemically defined nutrient media
- Pre-adjusted to the appropriate pH and osmolality
- Require only standard supplementation with L-glutamine for L-glutamine dependent systems
- Can be customized for modified catalog and customers' proprietary formulations

Granular format
- Dissolves instantly for faster media preparation times
- Dust-free granules reduce mess and environmental contamination
- Performance equivalent to liquid media
- Workflow improvement
- Helps reduce total cycle costs due to lower number of components, decreasing time involved in raw material planning, procurement, and testing
- Faster and easier media preparation, with less potential for error
- Easy to scale up from research through production, in batch sizes from 2 kg to 6,000 kg
- Available in various packaging options

What is AGT?

Granulated cell culture media is an easy-to-use, granular form of dry media that is produced by applying fluid bed granulation technology to cell culture nutrient media manufacturing. The patented process allows production of complete formulations of a variety of serum-free, protein-free, and chemically defined media in a dry format.

AGT offers the benefits of liquid media without the cost, storage, and transportation issues. It is a complete medium that is pH and osmolality pre-adjusted. The granules dissolve instantly for faster media preparation time than conventional dry powder media. AGT media can therefore help reduce total cycle costs, decreasing time involved in raw material planning, procurement, and testing as well as media preparation. These benefits are all realized while maintaining comparable cell growth to liquid media.

For Research Use Only. Not for use in diagnostic procedures.