Recombinant Protein;Human CD19 (20-291) Protein, Fc Tag, low endotoxin (Super affinity);1MG;Human CD19 (20-291), Fc Tag, low endotoxin (Super affinity) (CD9-H5251) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 56.6 kDa. The protein migrates as 70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized FMC63 at 1 μg/mL (100 μL/well) can bind Human CD19 (20-291), Fc Tag, low endotoxin (Super affinity) (Cat. No. CD9-H5251) with a linear range of 1-31 ng/mL (QC tested).;Human CD19 (20-291), Fc Tag, low endotoxin (Super affinity) (Cat. No. CD9-H5251) captured on CM5 chip via Anti-Human lgG Fc antibodies surface, can bind FMC63 MAb (mouse lgG2a) with an affinity constant of 0.17 nM as determined in a SPR assay (Biacore T200) (Routinely tested).;293 cells were transfected with FMC63-scFv and RFP tag. 2e5 of th