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ACROBiosystems Recombinant Protein;Human CD19 (20-291) Protein, His Tag;HEK293;50UG;CD9,CD19,CD-19,B4,B-4,CVID3,CVID-3,MGC12802

Supplier:  ACROBiosystems CD9H52H250UG

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Recombinant Protein;Human CD19 (20-291) Protein, His Tag;50UG;Human CD19, His Tag (CD9-H52H2) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 32.0 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized FMC63 MAb at 2 μg/mL (100 μL/well) can bind Human CD19, His Tag (Cat. No. CD9-H52H2) with a linear range of 10-78 ng/mL (QC tested).;FMC63 MAb (mouse lgG2a) captured on CM5 chip via anti-mouse antibodies surface, can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with an affinity constant of 2.95nM as determined in a SPR assay (Biacore T200) (Routinely tested).;293 cells were transfected with FMC63-scFv and RFP tag. 2x105 of the cells were first incubated with A. His Tag-protein control. B. Recombinant human CD19, His Tag (Cat. No. CD9-H52H2, 10 μg/ml). C. Recomb

Catalog No. 50-165-8229


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