Invitrogen™ Human CD226/DNAM-1 ELISA Kit

Description

ELISA

The Human CD226/DNAM-1 ELISA quantitates Hu CD226 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD226. Principle of the method The Human CD226 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

DNAM 1 is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes.

Specifications

• Accession Number: Q15762
• Assay Range: 6.14 to 600 ng/mL
• Assay Sensitivity: 5 ng/mL
• Calibration Range: 5 ng/mL to 600 ng/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: CD Protein
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects human DNAM1. Other species not determined.
• Sufficient For: 5 x 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 10666
• Gene Symbol: DNAM-1,DNAM1,PTA1,TLiSA1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: DNAM-1, DNAM1, PTA1, TLiSA1
• Target: CD226 (DNAM-1)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins