Recombinant Protein;Human / Cynomolgus / Rhesus macaque CD28 Protein, Fc Tag (HPLC-verified);1MG;Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (CD8-H525a) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (Cat. No. CD8-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-2, Fc Tag (Cat. No. CD6-H82F5) with a linear range of 0.019-0.625 μg/mL (QC tested).;Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (Cat. No. CD8-H525a) at 2 μg/mL (100 μL/well) can bind Anti-Mouse CD28 MAb with a linear range of 0.1-2 ng/ml (QC tested).