Mouse CD3 epsilon, also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. Together with other subunits and the T-cell receptor, it forms the T cell receptor-CD3 complex, which is essential for coupling antigen recognition to intracellular signal-transduction pathways and T-cell development.
- Expressed from HEK293 cells with a human IgG1 Fc tag at the C-terminus.
- Purity of >95% as determined by SDS-PAGE.
- Endotoxin level is <1.0 EU per μg by the LAL method.
- Calculated molecular weight of 36 kDa, migrating as 40–43 kDa under reducing conditions due to glycosylation.
- Lyophilized from a 0.22 μm filtered solution in Tris with Glycine, Arginine, and NaCl, pH 7.5 with trehalose as a protectant.
- Recommended for storage at -20°C or lower in lyophilized state.