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ACROBiosystems Recombinant Protein;Human CD40 / TNFRSF5 Protein, Fc Tag (HPLC-verified);HEK293;1MG;CD0,CD40,CD-40,Bp50,Bp-50,CDW40,MGC9013,TNFRSF5,TNFRSF-5,p50,p-50
Supplier: ACROBiosystems CD0H52531MG
Recombinant Protein;Human CD40 / TNFRSF5 Protein, Fc Tag (HPLC-verified);1MG;Human CD40, Fc Tag (CD0-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # NP_001241.1).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 45.3 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human CD40 Ligand, His Tag (Cat. No. CDL-H5248) at 5 μg/mL (100 μL/well) can bind Human CD40, Fc Tag (HPLC-verified) (Cat. No. CD0-H5253) with a linear range of 0.039-1.25 μg/mL (QC tested).;FACS assay shows that recombinant Human CD40, Fc Tag (HPLC-verified) (Cat. No. CD0-H5253) can bind to 293T cells overexpressing human CD40L, and the binding is inhibited by neutralizing Anti-Human CD40 MAb. The concentration of CD40 and Anti-Human CD40 MAb used are 0.03 μg/ml and 0.3 μg/ml respectively.
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