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ACROBiosystems ACROBiosystems Human CD73 / NT5E Protein, His Tag (HPLC-verified)
Supplier: ACROBiosystems CD3H52H7500UG
Recombinant Protein;500UG;Human CD73, His Tag (HPLC-verified) (CD3-H52H7) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.9 kDa. The protein migrates as 30 kDa, 40 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD73 Antibody, Human IgG1 with a linear range of 0.15-5 ng/mL (Routinely tested).;Anti-CD73 antibody (Human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) with an affinity constant of 0.164 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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