Recombinant Protein;25UG;Biotinylated Human CD73, His,Avitag (CD3-H82E3) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 61.7 kDa. The protein migrates as 42 kDa and 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Monoclonal Anti-Human CD73 Antibody, Human IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD73, His,Avitag (Cat. No. CD3-H82E3) with a linear range of 0.6-5 ng/mL (QC tested).;Immobilized Biotinylated Human CD73, His,Avitag (Cat. No. CD3-H82E3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD73 Antibody, Human IgG1 with a linear range of 0.3-2 ng/mL (Routinely tested).