Novus Biologicals™ CD8 Antibody Pair [HRP]

Monoclonal Antibody Pair

Manufacturer:  Novus Biologicals™ NBP27961215PLATES

Catalog No. NB010263

Description

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Ferret CD8.

Specifications

• Assay Range: 78 pg/mL
• Format: Conjugated
• Gene ID (Entrez): 925
• Product Type: Antibody Pairs
• Sensitivity: 78-5000 pg/mL
• Storage Requirements: Storage is content dependent.
• Host Species: Rabbit/Mouse
• Quantity: 15 Plates
• Assay: Sandwich ELISA
• Gene Alias: CD8, CD8a molecule, LEU2, p32

• Conjugate: HRP
• For Use With (Application): Sandwich ELISA
• Kit Contents:
• Standard: Each vial contains 110 ng of recombinant CD8.
• Capture Antibody: 1 mg/mL of rabbit anti-CD8 monoclonal antibody (in PBS, pH 7.4).
• Detection Antibody: 0.2 mg/mL of mouse anti-CD8 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Regulatory Status: RUO
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Ferret CD8.
• Target: CD8
• Research Discipline: Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Phospho Specific, Signal Transduction, Stem Cell Markers
• Target Species: Ferret
• Gene Symbol: CD8A
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.