Invitrogen™ Human CDK2/cyclin E1, GST Tag Recombinant Protein

Catalog No.	Quantity
PV6296	100 μg
PV6295	10 μg
PV6297	1 mg

Catalog No. PV6296 Supplier Invitrogen™ Supplier No. PV6296

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Protein Form: Recombinant The gene accession number for the subunits CKD2 and Cyclin E1 are NP_001789.2 and NP_001229.1.

CDK2, or Cyclin-Dependent Kinase 2, is a critical member of the cyclin-dependent kinase family, which regulates cell cycle progression. CDK2 is particularly important for the transition from the G1 phase to the S phase and for the progression through the S phase of the cell cycle. Cyclin E is a regulatory subunit that binds to CDK2 and activates its kinase activity. The binding of Cyclin E to CDK2 is necessary for the proper functioning of CDK2, as cyclins regulate the activation and specificity of CDKs. Together, the CDK2/Cyclin E complex plays a pivotal role in cell cycle regulation by phosphorylating key substrates, including the retinoblastoma protein (Rb), which leads to the activation of E2F transcription factors and the initiation of DNA replication.

Specifications

• Accession Number: P24864, P24941
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 150mM NaCl, 0.1mM PMSF, 0.25mM DTT, 0.1mM EDTA, 25% glycerol, 10mM glutathione and no preservative; pH 7.5
• Gene ID (Entrez): 1017, 898
• Molecular Weight (g/mol): 60.2-73.4 kDa
• Name: Human CDK2/cyclin E1, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: CDK2: p33 (CDK2) CyclinE1: CCNE1, CCNE
• Common Name: CDK2/Cyclin E1
• Gene Symbol: CCNE1, CDK2
• Protein Length: 1-298
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 1-298
• Content And Storage: Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Kinase Group: Serine/Threonine Kinase
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Full Length
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Drug Discovery: Cell Cycle & Proliferation, Signal Transduction