Invitrogen™ Human CDK3/cyclin E1, GST Tag Recombinant Protein

Catalog No.	Quantity
PV6300	1 mg
PV6298	10 μg
PV6299	100 μg

Catalog No. PV6300 Supplier Invitrogen™ Supplier No. PV6300

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Protein Form: Recombinant The gene accession number for the subunits CDK3 and Cyclin E1 are NP_001249.1 and NP_001229.1.

CDK3, or Cyclin-Dependent Kinase 3, is a member of the cyclin-dependent kinase family involved in regulating cell cycle progression. Although less well-studied than some other CDKs, CDK3 is believed to play a role in the transition from the G1 phase to the S phase of the cell cycle. Cyclin E is a regulatory subunit that binds to CDK3 and activates its kinase activity. The binding of Cyclin E to CDK3 is crucial for the proper functioning of CDK3, as cyclins regulate the activation and specificity of CDKs. Together, the CDK3/Cyclin E complex contributes to the regulation of the cell cycle by phosphorylating target proteins that are essential for the G1 to S phase transition, thereby promoting DNA replication and cell division.

Specifications

• Accession Number: P24864, Q00526
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 25% glycerol, 0.1mM EDTA, 0.1mM PMSF, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA and no preservative; pH 7.5
• Gene ID (Entrez): 1018, 898
• Molecular Weight (g/mol): 61.3-73.4 kDa
• Name: Human CDK3/cyclin E1, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: CDK3: None CyclinE1: CCNE1, CCNE
• Common Name: CDK3/Cyclin E1
• Gene Symbol: CCNE1, CDK3
• Protein Length: 1-305
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 1-305
• Content And Storage: Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Kinase Group: Serine/Threonine Kinase
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant
• Form: Liquid