Invitrogen™ Human CDK8/Cyclin C, His Tag Recombinant Protein

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Full Length

CDK8 phosphorylates the carboxy-terminal domain of the largest subunit of RNA polymerase II. This protein is coexpressed and copurified with Cyclin C. CDK8 is part of a family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34/Cdc 2, and a related protein Cdk 2, function late in the cycle while Cdk 4 and Cdk 6 are critically involved in G1 to S progression. Because of analogy with yeast Cdk 8, it has been proposed that the human Cdk 8 Cyclin C may interact with RNA polymerase, thus influencing the transcription mechanism(s) that lead to cell division.

Specifications

• Accession Number: P49336
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50 mM tris with 0.02% Triton X-100, 0.5 mM EDTA, 150 mM NaCl, 2 mM DTT, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 1024
• Molecular Weight (g/mol): 97 kDa
• Name: Human CDK8/Cyclin C, His Tag
• Quantity: 10 μg
• Gene Alias: Cdk8; CDK8 protein kinase; cell division protein kinase 8; Cell division protein kinase 8-like protein; cyclin dependent kinase 8; cyclin-dependent kinase 8; K35; mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; MGC126074; MGC126075; protein kinase K35; RGD1560888; wu:fk95h11
• Common Name: CDK8
• KinaseFamily: CDK and CHK Kinase Family
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical binding/kinetic
• Kinase Group: Ser⁄Thr Kinases: CMCG Group
• Shipping Condition: Dry Ice
• Target Entry: CDK8
• Target Gene: cyclin-dependent kinase 8
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Full Length
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Cell Cycle & Proliferation, Signal Transduction
• Purity or Quality Grade: 80% by SDS-PAGE

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov