Invitrogen™ Human CEACAM1 (BGP) ELISA Kit

Catalog No.	Quantity
EHCEACAM1X5	5 x 96 Tests
EHCEACAM1	96 Tests

Catalog No. EHCEACAM1X5 Supplier Invitrogen™ Supplier No. EHCEACAM1X5

Description

ELISA

The Human CEACAM1 (BGP) ELISA quantitates Hu CEACAM1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CEACAM1. Principle of the method The Human CEACAM1\ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CEACAM1 is a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of only two has been determined.

Specifications

• Accession Number: P13688
• Assay Range: 20.58-15000 pg/mL
• Assay Sensitivity: 20 pg/mL
• Calibration Range: 20 pg/mL to 15,000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Sufficient For: 5 x 96 Assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: antigen CD66; ATP-dependent taurocolate-carrier protein; bb-1; BGP; BGP1; BGP-1; BGPI; BGPR; Biliary glycoprotein 1; biliary glycoprotein D; bone gamma-carboxyglutamic acid (Gla) protein; carcinoembryonic antigen 1; carcinoembryonic antigen 7; carcinoembryonic antigen related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); Cc1; C-CAM; C-CAM 105; Ccam1; CD66a; CD66a antigen; Cea1; Cea-1; Cea7; Cea-7; CEACAM1; CEA-related cell adhesion molecule 1; CEA-related cell adhesion molecule 1 (bone gamma-carboxyglutamic acid (Gla) protein) (osteocalcin); Cell-CAM 105; ecto-ATPase; GP110; hepatitis virus (MHV-4) susceptibility; hepatitis virus receptor; Hv2; Hv-2; mCEA1; Mhv-1; MHVR; MHV-R; MHVR1; mmCGM1; mmCGM1a; mmCGM2; murine hepatitis virus receptor; osteocalcin; pp120; pp120/ecto-ATPaseantigen CD66; ATP-dependent taurocolate-carrier protein; bb-1; BGP; BGP1; BGP-1; BGPI; BGPR; Biliary glycoprotein 1; biliary glycoprotein D; bone gamma-carboxyglutamic acid (Gla) protein; carcinoembryonic antigen 1; carcinoembryonic antigen 7; carcinoembryonic antigen related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); Cc1; C-CAM; C-CAM 105; Ccam1; CD66a; CD66a antigen; Cea1; Cea-1; Cea7; Cea-7; CEACAM1; CEA-related cell adhesion molecule 1; CEA-related cell adhesion molecule 1 (bone gamma-carboxyglutamic acid (Gla) protein) (osteocalcin); Cell-CAM 105; ecto-ATPase; GP110; hepatitis virus (MHV-4) susceptibility; hepatitis virus receptor; Hv2; Hv-2; mCEA1; Mhv-1; MHVR; MHV-R; MHVR1; mmCGM1; mmCGM1a; mmCGM2; murine hepatitis virus receptor; osteocalcin; pp120; pp120/ecto-ATPase; BGP; BGP1; BGPI
• Gene ID (Entrez): 634
• Gene Symbol: CEACAM1
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Biliary glycoprotein 1, BGP-1
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Angiogenesis
• Sample Volume: Plasma 3.3 μL, Serum 3.3 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: BGP, BGP1, BGPI
• Target: CD66a (CEACAM1)
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min