Ceftiofur sodium (CAS No 104010-37-9) is a broad-spectrum cephalosporin antibiotic primarily used in veterinary medicine It exerts its antibacterial effects by inhibiting bacterial cell wall synthesis specifically targeting the penicillin-binding proteins which leads to cell lysis and death Ceftiofur sodium is active against a wide range of Gram-positive and Gram-negative bacteria with its in vitro efficacy generally observed in the nanomolar to low micromolar range This compound is commonly utilized in various research applications including studies using cell line models and animal studies to assess antibacterial efficacy resistance patterns and pharmacokinetics It also serves as a valuable tool in drug screening assays aiming to develop and test new antimicrobial strategies Experimental concentrations typically depend on the specific research design and objectives