Sigma Aldrich Fine Chemicals Biosciences Cell Proliferation Kit I M

Supplier:  Sigma Aldrich Fine Chemicals Biosciences 11465007001

The Cell Proliferation Kit I (MTT) is a colorimetric assay for the nonradioactive quantification of cellular proliferation viability and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates.Colorimetric assays analyze the number of viable cells by the cleavage of tetrazolium salts added to the culture medium. This technique requires neither washing nor harvesting of cells and the complete assay from microculture to data analysis by an ELISA reader is performed in the same microplate.MTT was the first tetrazolium salt described. It is cleaved to formazan by enzymes of the endoplasmic reticulum. This bioreduction occurs in viable cells only and is related to NAD(P)H production through glycolysis. Therefore the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.Colorimetric assay (MTT based) for the nonradioactive quantification of cellular proliferation viability and cytotox

Catalog No. 50-290-6115