ToLuminate PEG Photogel Kit photo-crosslinkable hydrogel DESCRIPTION The ToLuminate PEG Photogel Kit manufactured by Cellendes GmbH provides reagents for setting up cell-compatible hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry Its major components are norbornene-modified dextran (N-Dextran) and the thiol-modified cross-linker PEG-Link When the two reagents are combined and illuminated in the presence of LAP (Lithium phenyl-2 4 6-trimethyl benzoyl phosphinate) thiol groups on PEG-Link are activated and form stable thioether bonds with norbornene groups on N-Dextran The components are mixed at physiological pH (pH 7 2) for optimal cell compatibility There is ample time to place the pre-gel solution in any culture dish or container since gelation starts only after illumination Cell adhesion peptides can be covalently attached to N-Dextran to provide a cell-adhesive matrix QUANTITY Up to 4 ml hydrogel