1CellPlate is a 96-well plate based single-cell isolation device integrated with microfluidics channels. It is made of polystyrene and arranges wells as the standard well plate. The operation is by pipetting cells into the center well and distributing through small channels to the satellite wells, to form single-cell aliquots in less than 2.5 µL volumes. Features: 1) It provides rapid, selective, and damage-free cell isolation. 2) It simplifies steps for harvesting target cells and genes. 3) It is compatible with all cell sizes smaller than 80 µm at either low or high cell density. 4) It uniformly distributes cell suspension into 32 outlet wells and retrieves single target cell by pipetting. 5) The isolated single cells can be cultured in the plate to generate clonal cells. 6) It has 3 repeating units.Parameters: 1) Size: standard 96-well plate. 2) Well volume: 2.7 µL center well