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Sigma Aldrich Fine Chemicals Biosciences CelLyticTM NuCLEARTM Extra
Supplier: Sigma Aldrich Fine Chemicals Biosciences NXTRACT1KT

The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted the cytoplasmic fraction is removed and the nuclear proteins are released from the nuclei by a high salt buffer.
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