Biotium CF488A and 7-AAD Apoptosis Assay Kit

Supplier:  Biotium 30060

This kit contains CF488A Annexin V for staining apoptotic cells green, and 7-AAD for staining necrotic cells with far-red fluorescence, for detection by flow cytometry or fluorescence microscopy. Annexin V labeled with CF488A (Abs/Em= 490/515 nm) can identify apoptotic cells in green by binding to phosphatidylserine (PS) exposed on the outer leaflet. Our CF488A dye is superior to fluorescein/FITC as it is not affected by pH and has much better photostability. 7-AAD is a membrane-impermeant nucleic acid dye that is excluded by live cells, but stains necrotic cells and late apoptotic cells. 7-AAD (Abs/Em with DNA = 543/655 nm) emits in the far-red spectrum and can be well separated from CF488A and other green emitting dyes using the 488 nm line of a flow cytometer. The kit has reagents sufficent for 100 assays.

Catalog No. 50-196-4487