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Apexbio Technology LLC CFDA-SE(Synonyms: CFSE, Carboxyfluorescein succinimidyl ester, 5-(and-6)-Carboxyfluorescein diacetate succinimidyl ester), 10mg, CAS: 150347-59-4.

Supplier: Apexbio Technology LLC C343010
CFDA-SE (Carboxyfluorescein diacetate succinimidyl ester CAS 150347-59-4) is a membrane-permeant fluorescent dye commonly utilized for labeling viable cells and monitoring cellular proliferation Upon entering cells intracellular esterases hydrolyze CFDA-SE into carboxyfluorescein succinimidyl ester (CFSE) which becomes fluorescent (excitation 494 nm emission 521 nm) binds covalently to intracellular amino groups and is thereafter trapped within the cell cytoplasm Each cell division results in halving fluorescence intensity enabling accurate quantification of successive proliferation events via flow cytometry CFDA-SE labeling methodology has extensive applications in assessing lymphocyte fibroblast NK cell and bacterial proliferation both in vitro and in vivo
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