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Cayman Chemical cGAS InhIbItr Screeng as 96Wel
Supplier: Cayman Chemical 70193096 WELL
Cayman’s cGAS Inhibitor Screening Assay Kit provides a robust and EAsy-to-use platform for identifying novel inhibitors of human cGAS, which is a cytosolic dsDNA sensor that detects microbial pathogens. The assay directly measures 2’3’-cGAMP produced by cGAS in the presence of DNA, ATP, and GTP. The cyclic dinucleotide product of that reaction is quantified via ELISA using a 2’3’-cGAMP-specific antiserum. The cGAS inhibitor CU-76 is included as a positive control. The ELISA portion of Cayman’s cGAS Inhibitor Screening Assay Kit is a competitive assay with a range of 4.57-10,000 pM, a midpoint of approximately 300 pM (50% B/B0), and a sensitivity (80% B/B0) of approximately 50 pM.
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