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Invitrogen™ Chargeswitch™ Pro ET Removal Wash Buffer

Catalog No. CS31110
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Catalog No. CS31110 Supplier Invitrogen™ Supplier No. CS31110
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Description

Chargeswitch™ Pro ET Removal Wash Buffer is a component in the Chargeswitch™ Pro Filter Plasmid kits.

Chargeswitch™ Pro ET Removal Wash Buffer is a component in the Chargeswitch™ Pro Filter Plasmid kits. With the endotoxin removal buffer, the endotoxin levels of <10 EU/ug can be achievable and it can be used in all Chargeswitch™ Pro plasmids.

TRUSTED_SUSTAINABILITY

Specifications

Buffer Wash Buffer
Content And Storage 500 mL Wash Buffer. Store (at room temperature)
Isolation Technology ChargeSwitch Chemistry
Sample Type DNA (Plasmid)
Final Product Type Plasmid DNA
High-throughput Compatibility Not High-throughput Compatible (Manual)
Product Line ChargeSwitch
Product Type Pro ET Removal Wash Buffer
Quantity 1 each
Shipping Condition Room Temperature
System Type ChargeSwitch™
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Frequently Asked Questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.
What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.

For Research Use Only. Not for use in diagnostic procedures.

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