MilliporeSigma™ Chemicon™ Embryonic Stem Cell Characterization Kit

Description

Used in Immunocytochemistry applications. Chemicon™ Embryonic Stem Cell Characterization Kit is a specific and sensitive tool for phenotypic assessment of the differentiation status of ES cells by measuring AP activity, cell-surface stage-specific antigens (SSEA-1, SSEA-4), expression of TRA-1-60, TRA-1-81 antigens.

• Consists of two components used for alkaline phosphatase activity determination as well as four ES cell-specific antibodies required to perform 100 tests (including controls)
• Species Reactivity: Human, Mouse, Rat

Specifications

• Cell Type: Human Embryonic Stem Cells, Mouse Embryonic Stem Cells, Induced Pluripotent Stem Cells
• Product Type: Stem Cell Characterization Kit
• For Use With (Application): Immunocytochemistry
• Quantity: 100 Assays
• Regulatory Status: RUO
• Content And Storage:
  • Fast Red Violet solution (0.8g/L stock) (Part No. 90239). One 15mL bottle.
  • Napthol AS-BI phosphate solution (4mg/mL) in AMPD buffer (2mol/L), pH 9.5 (Part No. 90234). One 15mL bottle.
  • MS X SSEA-1, IgM, clone MC-480 (Part No. 90230). One vial containing 100μl of 1mg/mL monoclonal antibody.
  • MS X SSEA-4, IgG, clone MC-813-70 (Part No. 90231). One vial containing 100μl of 1mg/mL monoclonal antibody.
  • MS X TRA-1-60, IgM, clone TRA-1-60 (Part No. 90232). One vial containing 100μl of 1mg/mL monoclonal antibody
  • MS X TRA-1-81, IgM, clone TRA-1-81 (Part No: 90233). One vial containing 100μl of 1mg/mL monoclonal antibody.
  When stored at 2 to 8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.

For Research Use Only