MilliporeSigma™ Chemicon™ ESGRO Complete PLUS Clonal Grade Medium

Description

Provided with selective GSK3β inhibitor to enhance viability of mouse embryonic stem cells and increased maintenance of pluripotency in the absence of serum and feeder cells. Chemicon™ ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium intended for mouse ES cell expansion and growth at clonal density in serum-free conditions.

• Can be used on feeder dependent/independent ES cell lines
• Components:
• ESGRO Complete Clonal Grade Medium: (Catalogue No. SF001-B) One (1) 500mL bottle containing original clonal grade medium. Complete formulation containing self-renewal factors, no need to add LIF. pH 7.1 to 7.3
• GSK3β Inhibitor Supplement: (Catalogue No. SF012-250) One (1) 250μL vial containing selective GSK3β inhibitor

Specifications

• Form: Liquid
• Product Type: Stem Cell Media
• For Use With (Application): Stem Cell Culture, ES Cell derivation, Transfection and selection, General cell passaging and growth
• Quantity: 500 mL
• Regulatory Status: RUO
• Content And Storage: Upon receipt, store clonal grade medium and supplement in the dark at -20°C. Refer to lot expiration date on labels. Thawed clonal grade medium should be stored in the dark at 4°C for one month. Do not refreeze. Supplement may be thawed at room temperature, aliquoted and stored in the dark at 4°C for two months. After supplement is added to clonal grade medium, store in the dark at 4°C for up to two weeks. Do not refreeze.