MilliporeSigma™ Chemicon™ Human iPS Selection Kit

Description

Allows live cell imaging and identification of fully reprogrammed human induced pluripotent Stem cells from heterogeneous population of reprogramming intermediates. MilliporeSigma™; Chemicon™ Human iPS Selection Kit is a quick, easy and non-invasive method to monitor the pluripotent state of live human ESC and fully reprogrammed human iPS cells using immunocytochemistry (ICC).

• Enables live selection of human iPS cells that can be further passaged and expanded for downstream applications
• Selected live stained human iPS clones maintain normal human ESC morphology and can proliferate and be passaged normally
• Fully reprogrammed human iPS cells possess following characteristics:
• They downregulate expression of fibroblast marker, CD13
• They upregulate expression of pluripotent markers, SSEA-4 and TRA-1-60
• They silence viral transgenes
• Activates endogenous expression of Nanog
• Assume HoeschstDim phenotype
• Kit Components:
• Anti-SSEA-4 PE (Mouse monoclonal IgG3), Cat. No. CS204438. One vial containing 50μg of 0.25mg/mL purified mouse IgG antibody
• Anti-TRA-1-60 FITC (Mouse monoclonal IgM), Cat. No. CS204460. One vial containing 50μg of 0.5mg/mL purified mouse IgM antibody
• Anti-CD13 PE (Mouse monoclonal IgG1), Cat. No. CS204449. One vial containing 50μg of 1mg/mL purified mouse IgG antibody
• Hoechst Nuclear Stain, Cat. No. CS204269. One vial containing 1μL of 1mg/mL stock solution

Specifications

• Product Type: Human iPS Selection Kit
• For Use With (Application): Immunocytochemistry
• Quantity: 1 Kit (Sufficient for 40 Tests/Antibody)
• Regulatory Status: RUO
• Content And Storage: Stable for 6 months at 2°to 8°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Maintain refrigerated at 2° to 8°C, protected from light in undiluted aliquots.