MilliporeSigma™ Chemicon™ Mesenchymal Adipogenesis Kit

Description

Obtains 30% mature adipocytes from the rat bone marrow derived mesenchymal stem cells.

• Stem Cell Type: Mesenchymal Stem Cells

Specifications

• Cell Type: Mesenchymal Stem Cells
• Product Type: Stem Cell Adipogenesis Kit
• Quantity: 1 Kit
• Regulatory Status: RUO
• Content And Storage:
  • Dexamethasone Solution: (Catalog No. 90357) One vial containing 100μL of 10mM Dexamethasone in ethanol. Store at -20°C.
  • IBMX Solution: (Catalog No. 90355) One vial containing 250μL of 0.5M 3-isobutyl-1-methylxanthine (IBMX) in DMSO. Store at -20°C.
  • Insulin Solution: (Catalog No. 90356) Two vials containing 250μL of 10mg/mL recombinant human insulin. Store at -20°C.
  • Indomethacin Solution: (Catalog No. 2003626) Three vials containing 1mL of 10mM Indomethacin in methanol. Store at -20°C.
  • Oil Red O Solution: (Catalog No. 90358) One bottle containing 60mL 0.36% Oil Red O Solution in 60% isopropanol. Store at room temperature.
  • Hematoxylin Solution: (Catalog No. 2003732) One bottle containing 50mL Hematoxylin. Store at room temperature.
  Note: Kit components require two different storage temperatures. 
  Dexamethasone Solution, IBMX Solution, Insulin Solution and Indomethacin Solution should be stored at -20°C. Oil Red O and Hematoxylin Solutions should be stored at room temperature. Storage of Oil Red O Solution at -20°C may result in formation of insoluble precipitates and is not recommended. If Oil Red O solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter. 
  Precautions: Oil Red O stains skin and clothing. IBMX, Dexamethasone and Indomethacin are irritants and potentially toxic. DMSO is readily absorbed through the skin. Wear a lab coat and gloves when handling these solutions. Isopropanol is flammable. Keep solutions containing isopropanol (Oil Red O Solution) away from open flames.