MilliporeSigma™ Chemicon™ Mesenchymal Stem Cell Osteogenesis Kit

Description

Promotes osteogenic differentiation of mesenchymal stem cells. Contains all the reagents necessary to readily differentiate mesenchymal stem cells to an osteogenic lineage as assessed by Alizarin Red staining.

• Detection method: Colorimetric

Specifications

• Product Type: Stem Cell Osteogenesis Kit
• Quantity: 1 Kit
• Regulatory Status: RUO
• Content And Storage:
  • Dexamethasone Solution: (Catalog No. 90357) One vial containing 100μL of 10mM dexamethasone in ethanol. Store at -20°C.
  • Ascorbic Acid 2-Phosphate Solution: (Catalog No. 2004011) One vial containing 500μL of 100μM ascorbic acid 2-phosphate in water. Store at -20°C.
  • Glycerol 2- Phosphate Solution: (Catalog No. 2004010) Three vials containing 1mL of 1M glycerol 2-phosphate in water. Store at -20°C.
  • Collagen, Type I: (Catalog No. 2004013) One vial containing 150μg collagen Type I. Store at -20°C.
  • Vitronectin: (Catalog No. 2004012) One vial containing 150μg vitronectin. Store at -20°C.
  • Alizarin Red Solution: (Catalog No. 2003999) One bottle containing 50mL Alizarin Red Solution. Store at 2° to 8°C.
  Note: Kit components require two different storage temperatures.
  Dexamethasone Solution, Ascorbic Acid 2-Phosphate Solution, Glycerol 2- Phosphate Solution, Collagen Type I, and Vitronectin should be stored at -20ºC. Alizarin Red Solution should be stored at 2° to 8°C. 
  Precautions: Dexamethasone is an irritant and potentially toxic. DMSO is readily absorbed through the skin. Wear a lab coat and gloves when handling these solutions. Please refer to the Material Safety Data Sheet at for further precautions.