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Sigma Aldrich Fine Chemicals Biosciences ChemiKine Brain Derived Ne
Supplier: Sigma Aldrich Fine Chemicals Biosciences CYT306
ChemiKine Brain Derived Neurotrophic factor (BDNf) kit is a sandwich enzyme immunoassay (EIA) which measures BDNf.The kit will measureBDNf from human and rat. Test Principle With the ChemiKine BDNf assay system rabbit polyclonal antibodies generated against human BDNf are coated onto a microplate and are used to capture BDNf from a sample. BDNf specific biotin conjugated mouse monoclonal antibodies detect the captured BDNf. After addition of streptavidin-enzyme substrate and stop SOLUTIONon the amount of BDNf is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNf concentration i.e. the higher the OD the higher the BDNf concentration in the sample. Application ChemiKine BDNf kit is designed to measure the amount of BDNf in cell culture supernatants tissue homogenates and biological fluid (serum plasma and serum-free) samples from human and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates.
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