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BioVendor, LLC Chi3L1 Human Elisa BioVendor LLC
Supplier: BioVendor, LLC REH016R
The BioVendor Human CHI3L1 (Chitinase 3-like 1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CHI3L1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human CHI3L1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CHI3L1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CHI3L1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CHI3L1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. One kit (96 wells). Calibration range: 6.14-1,500pg/ml. Limit of detection: 6pg/ml. Other names: Chitinase 3-like 1, YKL-40.
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