The Chicken Thyroxine (T4) ELISA Kit is designed to quantitatively measure T4 levels in serum and plasma samples using a competitive assay format This kit detects T4 within a dynamic range of 20 ng/mL to 320 ng/mL with a sensitivity limit of 20 ng/mL ensuring precise detection across physiological and experimental concentrations The protocol requires a short incubation period of 1-5 hours and utilizes a modest sample volume of 50-100 L per test making it efficient for routine laboratory workflows Absorbance measurements at 450 nm enable accurate quantification supported by standardized controls and calibration curves Ideal for avian endocrine studies this kit provides reliable data for researchers investigating thyroid function metabolic disorders or hormone dynamics in chickens with applications spanning veterinary diagnostics and poultry health monitoring