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Cayman Chemical Cholesterol Fluorometric/Colorimetric Assay Kit, 1 ea
SDP

Catalog No. 502886817
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Cayman’s Cholesterol Fluorometric/Colorimetric Assay Kit provides a simple method for the sensitive quantitation of cholesterol in biological samples, such as plasma, serum, cell extracts, and tissue extracts.; The assay is based on an enzyme-coupled reaction that detects both free cholesterol and cholesteryl esters.; By excluding cholesterol esterase, only free cholesterol will be oxidized and therefore can be distinguished from total cholesterol.; In the presence of horseradish peroxidase, MaxiProbe reacts with hydrogen peroxide with a 1:1 stoichiometry to produce a compound, the fluorescence of which can be quantified at excitation and emission wavelengths of 530 and 590 nm, respectively.; Alternatively, its absorbance can be measured at 570 nm.; The fluorometric assay has a range of 0-20 μM with a lower limit of detection of 1 μM.; The colorimetric assay has a range of 0-100 μM with a lower limit of detection of 1 μM.

Catalog No. 50-288-6817 Supplier Cayman Chemical Supplier No. 100076401 EA
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Cayman’s Cholesterol Fluorometric/Colorimetric Assay Kit provides a simple method for the sensitive quantitation of cholesterol in biological samples, such as plasma, serum, cell extracts, and tissue extracts.; The assay is based on an enzyme-coupled reaction that detects both free cholesterol and cholesteryl esters.; By excluding cholesterol esterase, only free cholesterol will be oxidized and therefore can be distinguished from total cholesterol.; In the presence of horseradish peroxidase, MaxiProbe reacts with hydrogen peroxide with a 1:1 stoichiometry to produce a compound, the fluorescence of which can be quantified at excitation and emission wavelengths of 530 and 590 nm, respectively.; Alternatively, its absorbance can be measured at 570 nm.; The fluorometric assay has a range of 0-20 μM with a lower limit of detection of 1 μM.; The colorimetric assay has a range of 0-100 μM with a lower limit of detection of 1 μM.

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