Claudin 3 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA137469

Catalog No. 01-673-300

Description

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is small intestine tissue.

Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc. Alternatively spliced transcript variants encoding different isoforms have been found.

Specifications

• Antigen: Claudin 3
• Classification: Polyclonal
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: O15551, Q63400, Q9Z0G9
• Gene Symbols: CLDN3
• Immunogen: Synthetic peptide derived from the C-terminus of mouse Claudin-3
• Quantity: 1 mL
• Primary or Secondary: Primary
• Target Species: Chicken, Human, Mouse, Pig, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Gene: CLDN3
• Gene Alias: AI182374; C7orf1; claudin 3; claudin-3; CLDN3; clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-R2; CPE-receptor 2; CPETR2; hRVP1; integral membrane protein claudin-3; mRVP1; Rat ventral prostate.1 protein; Rat ventral prostate.1 protein homolog; RVP1; tight junction protein; ventral prostate.1 protein; ventral prostate.1 protein homolog; ventral prostate.1-like protein
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12739, 1365, 374029, 431781, 65130
• Content And Storage: -20°C or -80°C if preferred
• Form: Liquid