Thermo Scientific™ Clear Flat-Bottom Immuno Nonsterile 96-Well Plates

Catalog No.	Surface Treatment	Certified	Volume (Metric) Working	Barcode
14-245-153	Immulon 4 HBX	No	280 μL	No Barcode
14-245-78	Immulon 1 B	No	230 μL	No Barcode
14-245-61	Immulon 2 HB	No	280 μL	No Barcode
12-565-135	MaxiSorp	Yes	350 μL	No Barcode
12-565-136	MaxiSorp	No	350 μL	No Barcode
12-565-376	PolySorp	No	350 μL	No Barcode
12-565-377	MaxiSorp	Yes	350 μL	No Barcode
12-565-730	MediSorp	Yes	350 μL	No Barcode
14-962-23	MultiSorp	No	350 μL	No Barcode
12-565-138	PolySorp	No	350 μL	No Barcode
21-377-269	Universal Binding	No	330 μL	No Barcode
21-377-270	Enhanced Binding	Yes	330 μL	No Barcode

Catalog No. 14-245-153 Supplier Thermo Scientific™ Supplier No. 3855

Description

Choose from four binding surfaces of these 96-well plates for reliable and reproducible results in colormetric assays.

Choose from four binding surfaces for reliable and reproducible results in colormetric assays with Thermo Scientific™ Clear Flat-Bottom Immuno Nonsterile 96-Well Plates, which feature Thermo Scientific™ Nunc™, Immulon™ and Microtiter™ clear plates that are ideal for quantitative and qualitative solid phase immunoassays.

96-Well Solid Plates:

• Clear polystyrene
• Choice of four surfaces for optimal binding
• Alphanumerically labeled rows and columns

Surfaces:

• Thermo Scientific PolySorp, Immulon 1B, and Universal Binding (UB) are for adsorption of hydrophobic molecules.
• Thermo Scientific Medisorp and Immulon 2HB are slightly hydrophilic and bind a diverse range of biomolecules.
• Thermo Scientific MaxiSorp, Immulon 4 HBX, and Enhanced Binding(EB) are hydrophilic and ideal for antibody sandwich assays.
• Thermo Scientific MultiSorp is very hydrophilic and works well for glycans and water soluble proteins.

Ordering Information:

Please note that availability may vary by country. 467320, 456537, 460984 and 439454 include certificate

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

• Barcode: No Barcode
• Binding Property: Hydrophilic
• Certified: No
• Format: Standard
• Lid: Without Lid
• Detection Method: Colorimetric
• Array: 8 x 12
• Packaging: 50 Cases
• Binding Type: Hydrophilic
• Color: Clear
• Product Type: Microplate
• Material: Polystyrene
• Volume (Metric) Well: 330 μL
• No. of Wells: 96
• Volume (Metric) Working: 280 μL
• Sterility: Non-sterile
• Surface Treatment: Immulon 4 HBX
• Well Shape: Flat Bottom (F-well)
• No. per Case: 50
• No. per Pack: 10
• Plate Blocking: None
• Target Molecule: Antibodies (Glycoproteins)
• Well Design: F96

Frequently Asked Questions (FAQs)

Are your Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules compatible with all Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates?

Our Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules were designed to provide a positive seal for flat and round bottom wells of our Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates.
8-Well Strip Caps for Immuno Standard Modules are not compatible with C or V bottom wells of the Nunc Microwell 96-Well Microplates and Nunc Immuno 96-Well Plates.

For Nunc Immuno plates and modules, what are the advantages of one well geometry type over another? Which types should I use for various applications?

The following list describes the geometries of wells available for Nunc Immuno-plates and modules:
- Flat bottom (F): Allows maximum transmission of light. These plates can be read on a monochromatic reader.
- Round bottom (U): This geometry optimizes washing and coating. These plates can be read using a dual wavelength reader.
- "C" bottom (C): This design of the well is a combination of both flat and round bottoms. Basically, it is a flat bottomed well with curved edges at the bottom. These plates also can be read using a monochromatic reader combining optimal reading and washing.
- StarWell: These wells have a modified "C" shape geometry with eight fins strategically placed at the bottom. This feature increases surface area, allowing more molecules to become immobilized which reduces incubation times.

Which Nunc immunodiagnostic passive surface should I choose: PolySorp, MediSorp, MaxiSorp, or MultiSorp?

Our passive immunodiagnostic surfaces are based on a series of treatments that modify the polystyrene surface to different final levels of hydrophilicity. Selection of one of these surfaces for ELISA development should be based on the characteristics of the biomolecule you seek to immobilize. PolySorp is fairly hydrophobic and should be used for biomolecules that have hydrophobic domains, e.g., lipids, lipoproteins, and large proteins. MediSorp is slightly hydrophilic and can be used for biomolecules with hydrophilic and hydrophobic properties, e.g., medium-to-large proteins such as albumin, or amphiphilic biomolecules such as LPS. MaxiSorp is quite hydrophilic, optimized for binding of IgG (and any other molecule with similar characteristics) and thus is a popular choice for sandwich ELISA and similar protocols. MultiSorp is very hydrophilic, and in turn will immobilize very hydrophilic biomolecules such as glycoproteins. Some optimization will always be necessary, and in many cases screening for binding optimization may involve a few surface types.

What is the difference between a certified and an uncertified immunodiagnostic plate, e.g., Cat. No. 439454 vs. 442404?

There is no difference in the manufacturing process. Certified plates are performance tested (using an antibody binding protocol) during quality control, whereas uncertified plates are not. This test is expensive for us to perform, so we offer the uncertified version at cost savings customers for whom antibody binding testing results are not critical.