Invitrogen™ Click-iT™ EdU Colorimetric IHC Detection Kit

Catalog No.	Quantity
C10644	50 Slides

Catalog No. C10644 Supplier Invitrogen™ Supplier No. C10644

Description

Invitrogen™ Click-iT™ EdU Colorimetric IHC Detection Kit is a superior alternative to traditional methods for the colorimetric detection of proliferating cells within tissue sections.

• Substrate: DAB
• Substrate Type: HRP
• The Click-iT™ EdU Colorimetric IHC (Immunohistochemistry) Detection Kit is a superior alternative to traditional methods for the colorimetric detection of proliferating cells within tissue sections. In this assay, 5-ethynyl-2'-deoxyuridine (EdU), a nucleoside analog of thymidine containing an alkyne moiety, is incorporated into newly synthesized DNA. When biotin containing an azide group is added, a brief click reaction attaches the azide to the alkyne moiety on the EdU molecule. Upon addition of streptavidin–peroxidase and a peroxidase substrate, an enzymatic reaction generates a signal that can be detected with light microscopy and stored for future analysis.
• Optimized - enables colorimetric detection of proliferating cells in either tissue or cell samples
• Efficient - no denaturation steps or harsh treatment required
• Robust - improved preservation of cell morphology and the cellular environment offers content-rich results
• Consistent - not dependent on variable antibody lots for detection
• Simple - designed to work consistently, in less time than traditional methods

Specifications

• Conjugate: HRP
• Detection Method: Colorimetric
• For Use With (Equipment): EVOS™ XL Core Imaging System, EVOS™ XL Imaging System
• Quantity: 50 Slides
• Packaging: Slides
• Content And Storage: Store at 2°C to 8°C.
• Shipping Condition: Wet Ice
• Substrate: DAB
• Substrate Type: HRP
• Product Line: Click-iT
• For Use With (Application): IHC (Immunohistochemistry)
• Product Type: IHC Detection Kit
• Target Species: All species

Frequently Asked Questions (FAQs)

I have the Click-iT EdU Colorimetric IHC Detection Kit, but have decided that I want to use a fluorescent label but I don't want to buy another kit. What can I do?

You need to only substitute the peroxidase-conjugated streptavidin with a dye-conjugated streptavidin and not use the DAB. You would have to optimize the final concentration of the dye-conjugated streptavidin to use.

Can other peroxidase substrates be used with the Click-iT EdU Colorimetric IHC Detection Kit instead of the DAB?

Yes, but we have not optimized the amount of substrate to use. The labeling may be punctate and therefore an intense dark label such as DAB is recommended.

For Research Use Only. Not for use in diagnostic procedures.