Molecular Probes™ Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit

Catalog No.	Quantity
C10429	25 coverslips or two 96-well plates kit

Catalog No. C10429 Supplier Molecular Probes™ Supplier No. C10429

Description

Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit

The Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit provides a fast, sensitive, non-toxic, and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy, high-content imaging, or flow cytometry. Included in the kit are L-homopropargylglycine (HPG), an amino acid analog of methionine containing an alkyne moiety, and Alexa Fluor™ 594 azide. The HPG is fed to cultured cells and incorporated into proteins during active protein synthesis. Addition of the Alexa Fluor™ 594 azide leads to a chemoselective ligation or “click' reaction between the green fluorescent azide and the alkyne, allowing the modified proteins to be detected by imaged-based analysis.

Non-radioactive alternative—an alternative to the traditional ³⁵S-methionine

Visualize bulk protein dynamics—fluorescent tagging of proteins allows their localization to be determined, including aggregation

Specificity—selective, specific reaction between label and detection tags

Stability —product contains an irreversible, covalent bond

Multiplex-enabled—use in conjuction with Click™-iT AHA (azide amino acid and alkyne dye) to detect spatial and temporal differences

Applicability to biological samples—easy detection; high sensitivity and low background, regardless of complexity

The Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit has been successfully tested in HeLa, A549, and U-2 OS cells with a variety of reagents that inhibit protein synthesis, including cycloheximide and anisomycin. The applicability of these probes to monitor protein degradation has also been shown using inhibitors of the proteasome (MG132 and Bortezomib) and blockers of autophagy (chloroquine) in HeLa cells.

Additionally, due to differences in Click-iT™ chemistry between the Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit and Click-iT™ AHA Alexa Fluor™ 488 HCS Kit, these kits can be used in conjunction for spatial or temporal determination of differences in nascent protein synthesis.

Specifications

• Product Type: Protein Synthesis Assay Kit
• Quantity: 25 coverslips or two 96-well plates kit
• Content And Storage:
  • Store at 2°C to 6°C
  • DO NOT FREEZE
  • Protect material from long-term exposure to light, but may be exposed to light for short periods of time.
• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescence Microscope, Fluorescent Imager
• Product Line: Alexa Fluor, Click-iT
• Label or Dye: Alexa Fluor 594
• Format: Liquid
• Color: Red-Orange
• Labeling Target: Proteins
• Label Type: Alexa Fluor Dyes

For Research Use Only. Not for use in diagnostic procedures.