Invitrogen™ Click-iT™ sDIBO Alkynes for copper-free click chemistry

Description

Experience copper-free 'click chemistry' with Click-IT™ Alexa Fluor™, and sDIBO Alkyne reagents, which are ideal for surface labeling of live cells and minimize damage to enzymes and fluorescent proteins.

Achieve efficient labeling of azides with Click-It sDIBO Alkynes. Our Click-IT sDIBO alkynes enable the labeling of azido labeled targets without the need for copper catalysis and are available with a variety of Alexa Fluor or biotin labels, as well as amine or thiol reactive groups for creating your own conjugates. Macromolecules that have been azido-modified enzymatically, chemically, or metabolically can now be labeled easily, yielding soluble bioconjugates.

Click-iT sDIBO Alkyne labels react with azides via a copper-free Click chemistry reaction to form a stable triazole linkage with an Alexa Fluor, Biotin, or label of your choice. Copper-free click reactions using our sDIBO alkynes are ideal for surface labeling of live cells; they also minimize damage to fluorescent proteins like GFP or R-PE. For intracellular labeling, the nonspecific binding of DIBO alkynes creates a background signal, and a labeling strategy using picolyl azides is the preferred solution in these instances.

Click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be labeled more easily, yielding more soluble bioconjugates with improved biological labeling utility.

To make a concentrated stock solution from 2–5 mM, dissolve the dye-alkyne in high quality anhydrous DMSO. Store frozen, desiccated, and protected from light. Click-iT SDP Ester sDIBO alkyne is susceptible to hydrolysis, so prepare the stock solution fresh on the day of the experiment.

Specifications

• Green Features: Less hazardous
• Quantity: 1 mg
• Kit Contents: Contains 1 mg of SDP Ester sDIBO Alkyne
• Molecular Weight (g/mol): ∼1050
• Content And Storage: Store at ≤ -20°C. Desiccation recommended. Protect material from long-term exposure to light; may be exposed to light for short periods of time
• Labeling Method: Click Chemistry
• Shipping Condition: Dry Ice
• Reactive Moiety: SDP ester, sDIBO Alkyne
• Label or Dye: SDP ester
• Product Line: Click-iT™
• Labeling Target: Azide