Invitrogen™ Rat Clusterin (CLU) ELISA Kit

Catalog No.	Quantity
50-254-5398	96 Tests
50-254-5396	5 x 96 Tests

Catalog No. 50-254-5398 Supplier Invitrogen™ Supplier No. ERCLU

Description

ELISA

The Rat Clusterin (Rt CLU) ELISA quantitates Rt Clusterin in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt Clusterin. Principle of the method The Rat Clusterin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

Specifications

• Accession Number: P05371, Q06890
• Assay Range: 1.311-320 ng/mL
• Assay Sensitivity: 1.3 ng/mL
• Calibration Range: 1.3 ng/ml to 320 ng/ml
• Content And Storage: Pre-coated 96-well plate, standard, detector antibody, streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers. May be stored at 2–8°C for up to 6 months from the date of shipment.
• Description: Clusterin (CLU) ELISA Kit, 96 Assays, 1 Kit, Rat Species, Histones Protein Family, Oncology Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Clusterin (Clu) in Samples, Using a Microplate Reader
• Detectable Analytes: Clusterin (CLU)
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Histones
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects rat and mouse Clusterin. Other species not determined.
• Substrate Type: TMB
• Sufficient For: 96 Assays
• Target Species: Mouse, Rat
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AAG4; Aging associated protein 4; Aging-associated gene 4 protein; aging-associated protein 4; AI893575; apo j; Apo J a; Apo J alpha; Apo J b; Apo J beta; Apo J α; Apo J β; Apo Ja; Apo Jb; ApoJ; Apo-J; ApoJ a; ApoJ alpha; ApoJ b; ApoJ beta; ApoJ α; ApoJ β; ApoJa; ApoJalpha; ApoJb; ApoJbeta; Apolipoprotein J; CLI; Clu; CLU1; CLU2; clusterin; Clusterin alpha / beta chain; Clusterin alpha chain; Clusterin beta chain; Clustrin; Complement associated protein SP40; complement cytolysis inhibitor; Complement cytolysis inhibitor a chain; Complement cytolysis inhibitor b chain; complement lysis inhibitor; complement-associated protein SP-40,40; D14Ucla3; DAG; Dimeric acid glycoprotein; GP80; HGNC:2095; ku70-binding protein 1; Ku70-binding protein 1 (KUB1); KUB1; MGC24903; Msgp-2; NA1/NA2; RATTRPM2B; SGP2; Sgp-2; SP40; SP-40; Sugp-2; sulfated glycoprotein 2; Sulfated glycoprotein 2 (SGP2); testosterone repressed prostate message; testosterone repressed prostate message 2; Testosterone repressed prostate message 2 (TPRM2B); testosterone repressed prostate message-2; testosterone-repressed prostate message; testosterone-repressed prostate message 2; testostrone-repressed prostate message 2; Trpm2; TRPM-2; TRPM2B; TrpmbAAG4; Aging associated protein 4; Aging-associated gene 4 protein; aging-associated protein 4; AI893575; apo j; Apo J a; Apo J alpha; Apo J b; Apo J beta; Apo J α; Apo J β; Apo Ja; Apo Jb; ApoJ; Apo-J; ApoJ a; ApoJ alpha; ApoJ b; ApoJ beta; ApoJ α; ApoJ β; ApoJa; ApoJalpha; ApoJb; ApoJbeta; Apolipoprotein J; CLI; Clu; CLU1; CLU2; clusterin; Clusterin alpha / beta chain; Clusterin alpha chain; Clusterin beta chain; Clustrin; Complement associated protein SP40; complement cytolysis inhibitor; Complement cytolysis inhibitor a chain; Complement cytolysis inhibitor b chain; complement lysis inhibitor; complement-associated protein SP-40,40; D14Ucla3; DAG; Dimeric acid glycoprotein; GP80; HGNC:2095; ku70-binding protein 1; Ku70-binding protein 1 (KUB1); KUB1; MGC24903; Msgp-2; NA1/NA2; RATTRPM2B; SGP2; Sgp-2; SP40; SP-40; Sugp-2; sulfated glycoprotein 2; Sulfated glycoprotein 2 (SGP2); testosterone repressed prostate message; testosterone repressed prostate message 2; Testosterone repressed prostate message 2 (TPRM2B); testosterone repressed prostate message-2; testosterone-repressed prostate message; testosterone-repressed prostate message 2; testostrone-repressed prostate message 2; Trpm2; TRPM-2; TRPM2B; Trpmb; APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2; SP-40; SP40; TRPM-2; TRPM2B; Trpm2; Trpmb
• Gene ID (Entrez): 12759, 24854
• Gene Symbol: Clu
• Host Species: Rat
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Oncology
• Sample Volume: Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: APOJ, CLI, DAG, RATTRPM2B, SGP-2, SGP2, SP-40, SP40, TRPM-2, TRPM2B, Trpm2, Trpmb
• Target: Apolipoprotein J
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min