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Biotium Coelenterazine Sampler Kit
Supplier: Biotium 10123
For researchers wishing to screen for the appropriate coelenterazine derivatives, the sampler kit includes 25 ug each of nine coelenterazine analogs including native, cp, f, fcp, h, hcp, i, ip and n.Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator. It binds coelenterazine to form an aequorin complex that emits light upon calcium binding and measures concentrations ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter of transcription regulation and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines are also used for chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues. Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines are also available.Properties : Yellow solids soluble in MeOH or EtOH; Store at -20°C, protect from light.
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