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ANTIBODY DESIGN LABS CM13d3

Supplier: ANTIBODY DESIGN LABS PH040P

CM13d3 is a helper phage engineered for multivalent phage display. CM13d3 is a derivative of M13KO7 (1) having a wild-type pIII phenotype which allows efficient bacterial transduction but lacking a functional pIII gene thus driving the display of pIII-fusion proteins expressed by the phagemid on the phage head (2). The use of pIII-defective helper phage has been shown to increase display of scFv by a factor up to 100-fold and more (3-5). This preparation contains enough helper phage to superinfect up to 0.5 L (500 ml) of TG1 culture. Other bacterial strains may require different amounts of CM13d3 helper phage.
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