PAN-Biotech CMRL 1066 Cell Culture Medium, w/o: L-Glutamine, w/o: Phenol red, w: 2.2 g/L NaHCO3 (500 ml). BICARBONATE: With 2.2 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: Without glutamine. PHENOL RED: Without Phenol Red. HEPES: Without HEPES. SODIUM PYRUVATE: Without sodium pyruvate. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. INTRO: CMRL 1066 Medium is a chemically-defined medium, developed in the 1950s by Connaught Medical Research Laboratories for serum-free culture of L-strain cells, as a highly modified and less complex version of Medium 199 (M199). It has been used to maintain monolayer growth of permanent cell lines for years without protein supplementation. Initially developed for serum-free conditions, it can be supplemented with serum and used to support the growth of many cell types. COMPOSITION: CMRL-1066 is a nucleoside-rich, vitamin-rich medium that contains no proteins, lipids, or growth factors.