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Gibco™ CO2 Independent Medium


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Quantity:
500 mL
Description
- CO2 Independent Medium is a non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells such as epithelial, fibroblast, and lymphoid cell lines without a CO2 incubator
- CO2 Independent Medium is ideal for transporting cells or tissue, and for the handling of mouse embryos under atmospheric conditions
- Gibco™ CO2 Independent Medium contains a unique buffering system composed of mono and dibasic sodium phosphate and β-glycerophosphate
- A small amount of sodium bicarbonate has been included in the formulation to meet essential bicarbonate dependent functions
- No synthetic buffers are utilized, thus eliminating any cytotoxic effects associated with such buffering systems
- Additionally, Gibco™ CO2 Independent Medium has been formulated with components that enhance cellular production and utilization of CO2
- Store at 2°C to 8°C, protected from light
Modified as follows
- With: phenol red, sodium pyruvate
- Without: L-glutamine, HEPES
Using CO2 Independent Medium
- CO2 Independent Medium contains no lipids or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- The CO2 Independent Medium does not require a CO2 environment to maintain physiological pH
- For maximum growth performance some cell lines may require either direct or sequential adaptation to CO2 Independent Medium

Specifications
Specifications
Cell Line | Epithelial, fibroblast, and lymphoid |
Concentration | 1 X |
Form | Liquid |
Product Type | CO2 Independent Medium |
Sterility | Sterile-filtered |
With Additives | Phenol Red, Sodium Pyruvate |
Without Additives | No Glutamine, No HEPES |
Quantity | 500 mL |
Shipping Condition | Room Temperature |
Green Features | Sustainable packaging |
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Safety and Handling
WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
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