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Gibco™ CO2 Independent Medium
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Catalog No. 18045088
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18-045-088 500 mL
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Description

A non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells.

  • CO2 Independent Medium is a non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells such as epithelial, fibroblast, and lymphoid cell lines without a CO2 incubator
  • CO2 Independent Medium is ideal for transporting cells or tissue, and for the handling of mouse embryos under atmospheric conditions
  • Gibco™ CO2 Independent Medium contains a unique buffering system composed of mono and dibasic sodium phosphate and β-glycerophosphate
  • A small amount of sodium bicarbonate has been included in the formulation to meet essential bicarbonate dependent functions
  • No synthetic buffers are utilized, thus eliminating any cytotoxic effects associated with such buffering systems
  • Additionally, Gibco™ CO2 Independent Medium has been formulated with components that enhance cellular production and utilization of CO2
  • Store at 2°C to 8°C, protected from light

Modified as follows

  • With: phenol red, sodium pyruvate
  • Without: L-glutamine, HEPES

Using CO2 Independent Medium

  • CO2 Independent Medium contains no lipids or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • The CO2 Independent Medium does not require a CO2 environment to maintain physiological pH
  • For maximum growth performance some cell lines may require either direct or sequential adaptation to CO2 Independent Medium
TRUSTED_SUSTAINABILITY

Specifications

Cell Line Epithelial, fibroblast, and lymphoid
Concentration 1 X
Form Liquid
Product Type CO2 Independent Medium
Sterility Sterile-filtered
With Additives Phenol Red, Sodium Pyruvate
Without Additives No Glutamine, No HEPES
Quantity 500 mL
Shipping Condition Room Temperature
Green Features Sustainable packaging
Species Mouse Embryos
Content And Storage Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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