Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Gibco™ CO2 Independent Medium

CO2 Independent Medium


Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
View Documents
Each for $48.71
Add to cart


CO2 Independent Medium is a non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells such as epithelial, fibroblast, and lymphoid cell lines without a CO2 incubator. CO2 Independent Medium is ideal for transporting cells or tissue and for the handling of mouse embryos under atmospheric conditions.

Gibco™ CO2 Independent Medium contains a unique buffering system composed of mono and dibasic sodium phosphate and β-glycerophosphate. A small amount of sodium bicarbonate has been included in the formulation to meet essential bicarbonate dependent functions. No synthetic buffers are utilized, thus eliminating any cytotoxic effects associated with such buffering systems. Additionally, Gibco™ CO2 Independent Medium has been formulated with components that enhance cellular production and utilization of CO2 such that an exogenous source of CO2 is not required for the maintenance of CO2 dependent cellular functions.

Product Use
For research use only: Not intended for human or animal diagnostic or therapeutic uses.

Dual-Site cGMP Manufacturing
Gibco™ CO2 Independent Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical Gibco™ CO2 Independent Medium product made in our Scotland facility (18045-054). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

CO2 Independent Medium contains no lipids or growth factors. Therefore, CO2 Independent Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). The CO2 Independent Medium does not require a CO2 environment to maintain physiological pH. For maximum growth performance some cell lines may require either direct or sequential adaptation to CO2 Independent Medium (see protocol).

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Safety and Handling

Safety and Handling

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit