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Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Kits for Illumina Systems

Catalog No. p-7162472
Encompass_Preferred
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No. of Reactions:
24 Preps
96 Preps
Product Type:
PCR-free ES DNA Library Prep Core Kit
PCR-free ES DNA Library Prep Kit with CD Indexes
PCR-free ES DNA Library Prep Kit with UD Indexes (Set A, 1-24)
PCR-free ES DNA Library Prep Kit with UD Indexes (Set A-D, 1-96)
PCR-free ES DNA Library Prep Kit with UD Indexes (Set B, 25-48)
PCR-free ES DNA Library Prep Kit with UD Indexes (Set C, 49-72)
PCR-free ES DNA Library Prep Kit with UD Indexes (Set D, 73-96)

The Invitrogen Collibri PCR-free ES DNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes.

The Collibri PCR-free ES DNA Library Preparation Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about two hours for the PCR-free workflow. If needed, Collibri Library Amplification Master Mix with primers can be bought separately. Or see the Collibri ES DNA Library Preparation Kit, designed for fast and convenient library preparation with PCR from a wide range of inputs (1–500 ng).

The Collibri PCR-free ES DNA Library Preparation Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about two hours for the PCR-free workflow. If needed, Collibri Library Amplification Master Mix with primers can be bought separately. Or see the Collibri ES DNA Library Preparation Kit, designed for fast and convenient library preparation with PCR from a wide range of inputs (1–500 ng).

The Collibri PCR-free ES DNA Library Preparation Kit:
• Includes all necessary components in a single kit* for quick use
• Is empowered on miniaturization liquid handlers to cut on costs
• Provides in-process visual feedback for easier handling
•Is suitable for conventional automation to increase throughput

* The kit consists of enzymatic modification, size selection/clean-up, and indexed adapter-containing modules, except for the Core kit, which enables Collibri combination with desired indexed adapters

The Collibri PCR-free ES DNA Library Preparation Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. Moreover, the enzymatic shearing (ES) module of the kit generates a consistently high yield of library, which is enough for library quantification  and sequencing and multiple runs on Illumina instruments.

Applications
The Collibri PCR-free ES DNA Library Preparation Kit is recommended for:
Whole genome sequencing
• Resequencing
• De novo assembly

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • Collibri PCR-free ES DNA Library Prep Kit, store at -20°C
• Collibri DNA Library Cleanup Kit, store at 4°C
• Collibri DNA CD Indexes, store at -20°C
Description The product contains modules A38575024, A43190024
Format Kit
Sample Type DNA
Sequencing Type Genome and DNA Sequencing
For Use With (Equipment) HiSeq X, HiSeq2500, HiSeq3000, HiSeq4000, MiSeq or iSeq, NextSeq 550, NovaSeq 6000
For Use With (Application) Whole Genome Sequencing, Resequencing, De Novo Assembly
Libraries Fragment Library
No. of Reactions 24 Preps
Product Line Collibri™
Product Type PCR-free ES DNA Library Prep Core Kit
Quantity 24 preps
Shipping Condition Dry Ice
Workflow Step Library Generation
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