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BD CompBead Anti-Mouse Ig, κ/Negative Control Particles Set

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BDB552843 6 mL Each
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Description

Used to optimize fluorescence compensation settings for multicolor flow cytometric analyses. BD™ Anti-Mouse Ig, κ/Negative Control Particles Set includes two sets of polystyrene microparticles to distinctly stain positive and negative populations, especially when using tandem dye conjugates.

The set provides two populations of microparticles:

  • BD™ CompBeads Anti-Mouse Ig, κ—Binds any mouse κ light chain-bearing immunoglobulin
  • BD™ CompBeads Negative Control—Has no binding capacity

When mixed together with a fluorochrome-conjugated mouse antibody, the CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software.

Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra).

Use of the BD™ CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy™7, APC-Cy™7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.

TRUSTED_SUSTAINABILITY

Specifications

Antigen Mouse Ig, κ
Applications Flow Cytometry
Conjugate Unconjugated
Formulation CompBead Anti-Mouse Ig, kappa Particles
CompBead Negative Control (FBS) Compensation Particles
Purification Method Unpurified
Quantity 6 mL Each
Primary or Secondary Primary
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Form Liquid
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