Invitrogen™ Human Complement C5a ELISA Kit

Catalog No.	Quantity
50-112-5291	96 Tests

Catalog No. 50-112-5291 Supplier Invitrogen™ Supplier No. BMS2088

Description

ELISA

The Human Complement C5a (Hu C5a) ELISA quantitates Hu C5a in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C5a. Principle of the method The Human C5a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis.

Specifications

• Accession Number: P01031
• Assay Range: 0.078-5.0 ng/mL
• Assay Sensitivity: 0.005 ng/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with monoclonal antibody to human C5a
  Biotin-Conjugate anti-human C5a monoclonal antibody
  Streptavidin-HRP
  Human C5a Standard lyophilized, 5 ng/mL upon reconstitution
  Sample Diluent
  Calibrator Diluent
  Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  Substrate Solution (tetramethyl-benzidine)
  Stop Solution (1M Phosphoric acid)
  Adhesive Films
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: anaphylatoxin; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C4B1; C4B12; C4B2; C4B3; C4B5; C4F; C5; C5a; C5a anaphylatoxin; C5b; C5D; CH; CO4; complement C5; Complement C5 alpha chain; Complement C5 alpha' chain; Complement C5 beta chain; complement component 5; CPAMD3; CPAMD4; DADB-112B14.11; ECLZB; FLJ17816; FLJ17822; FLJ60561; Hc; He; Hemolytic complement; MGC142298; MGC164979; prepro-C5; RGD1561905anaphylatoxin; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C4B1; C4B12; C4B2; C4B3; C4B5; C4F; C5; C5a; C5a anaphylatoxin; C5b; C5D; CH; CO4; complement C5; Complement C5 alpha chain; Complement C5 alpha' chain; Complement C5 beta chain; complement component 5; CPAMD3; CPAMD4; DADB-112B14.11; ECLZB; FLJ17816; FLJ17822; FLJ60561; Hc; He; Hemolytic complement; MGC142298; MGC164979; prepro-C5; RGD1561905; C5Da; C5b; CPAMD4; ECLZB; C5
• Gene ID (Entrez): 727
• Gene Symbol: C5
• Interassay CV: 4%
• Intraassay CV: 3.6%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 5 μL, Supernatant 50 μL
• Storage Requirements: 2-8°C
• Synonym: complement component 5, C5a, C5D, C5b, ECLZB, CPAMD4
• Target: Complement C5a
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 30 min