Invitrogen™ Complement C5a Mouse ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EMHC

Catalog No. EMHC

Description

The Mouse Complement C5a (HC) ELISA quantitates Ms Complement C5a in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Compelemnt C5a. Principle of the method The Mouse Complement C5a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

C5 is part of the membrane attack complex (MAC) of the complement system. C5 is split into C5a og C5b by the C5 convertases from the classical and the alternative complement pathway and the C5b fragment initiates the assembly of the terminal MAC (C5b-C9). The molecular mass of C5 is 190 kDa.

Specifications

• Product Type: ELISA Kit
• For Use With (Equipment): Colorimetric Microplate Reader
• Assay: Solid-phase sandwich ELISA
• Conjugate: Biotin
• Accession Number: P06684
• Calibration Range: 1.5pg/mL to 400pg/mL
• Gene ID (Entrez): 15139
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Total Assay Time: 4 hr. 45 min.
• Research Discipline: Development
• Interassay CV: <12%
• Quantity: 96 Tests
• Sample Type: Plasma, Serum, Supernatant
• Gene Symbol: C5,C5a,He

• Sufficient For: 96 assays
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse BLC, CD27, CD30 Ligand, Eotaxin, Eotaxin-2, Fas Ligand, G-CSF, GM-CSF, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p40, IL-13, IL-15, IL-17, IL-21, KC, Leptin, LIX, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, PF-4, RANTES, TARC, TCA-3, TNF alpha, TNFRI, VEGF.
• Protein Family: Proteases, Peptidases & Inhibitors
• Label or Dye: HRP
• Assay Range: 1.64-100 pg/mL
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Sample Volume: Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL
• Test Time: 1 hr. 20 min.
• Target: Mouse
• Synonym: RP23-223C17.1, C5, C5a, He
• Intraassay CV: <10%
• Target Species: Mouse
• Assay Sensitivity: 1.5 pg/mL