This kit detects C3b in mouse serum plasma and tissue homogenates with high sensitivity and precision The assay has a detection range of 0 235 ng/mL to 15 ng/mL enabling accurate quantification of both low and high concentration samples With a sensitivity of 0 059 ng/mL it reliably identifies even minimal levels of C3b making it suitable for applications requiring precise measurement in limited sample volumes (50-100 L) Utilizing a sandwich ELISA format the kit provides robust and specific results within 1 to 5 hours The absorbance is measured at 450 nm ensuring clear and consistent readouts for quantitative analysis This optimized workflow balances speed and accuracy catering to diverse experimental needs in complement system research