Vector Laboratories Concanavalin A (Con A) Unconjugated 500 mg

Supplier:  Vector Laboratories L1000500

Concanavalin A (ConA) recognizes α-linked mannose residues present as part of a "core oligosaccharide" in many serum and membrane-bound glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer, but below pH 5.6, it dissociates into dimers of 52 kDa. Derivatizations can produce stable forms with dimeric structures, and nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds. Con A requires calcium or manganese ions at each binding site, so avoid using buffers that bind calcium (such as phosphate) to dilute ConA, since a gradual loss in activity may occur. This Con A is supplied as a solution preserved with sodium azide. The recommended inhibiting/eluting sugar is a mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside.

Catalog No. NC9054706