Concanavalin A, MP Biomedicals™

Catalog No.	Quantity
MP021507105	500 mg
MP021507102	250 mg

Catalog No. MP021507105 Supplier MP Biomedicals, Inc Supplier No. 02150710.5

Description

Isolated by affinity chromatography on cross-linked dextran. Concanavalin A, MP Biomedicals is pure Canavalia ensiformis lectin (Con A) from Jackbean. It exists as a dimer below pH 5.0, and at pH >7, it exists as a tetramer.

Lectin are used in a wide variety of applications. Con-A is not a glycoprotein and does not contain cysteine residues. Unlike most other lectins, Con-A is a metalloprotein and requires a transition metal ion, such as manganese, plus calcium ions for binding.

• Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Con-A binds specifically to mannosyl and glucosyl residues of polysaccharides and glycoproteins.
• Unmodified hydroxyl groups at the C3, C4 and C6 positions of D-glucopyranosyl or D-mannopyranosyl rings may be essential for binding.
• Each subunit of Con A contains one calcium ion and one manganese ion. Removal of these cations by dialysis under acidic conditions abolishes the carbohydrate-binding activity.
• Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer. Above pH 7.0 higher aggregates are formed.
• Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Specifications

Chemical Identifiers

• CAS: 11028-71-0
• Synonym: Con A

• Quantity: 500 mg
• Solubility Information: Soluble in water (10mg/mL, slightly hazy, colorless solution).
• Formula Weight: Monomeric molecular weight of Con-A is 25,500(Lit.)
• Physical Form: Powder

Safety and Handling

Recommended Storage

−20°C